A group of researchers under the leadership of camel Khalili from Templestowe University for the first time managed to remove from the genome of laboratory mice, the DNA segment of human immunodeficiency virus using a technology called CRISPR/Cas9. Tested approach is likely to contribute to the creation of new effective methods of treatment of HIV, experts hope.
The human immunodeficiency virus, or HIV, infects cells of the immune system and often leads to the development of AIDS, acquired immune deficiency syndrome . Like other retroviruses, HIV integrates its DNA into the genome of an infected person, thus becoming an integral part of the cell, which subsequently begins to reproduce copies of the virus.. today for the treatment of HIV infection are subject to special antiretroviral drugs. Although they can slow the spread of the virus, get rid of already infected cells by drug impossible.
In their study, the researchers tried to go a step further and using technology for genome editing CRISPR/Cas9 tried to remove all foreign DNA fragments from cells, while not exerting itself on her negative influence. Previously, similar experiments had already been carried out on culture cells taken from patients with HIV, however, in living organisms, the technology has so far not been tested. For the experiment, we used rats and mice, each cell of which contained the fragments of viral RNA. Experts have applied a protein called Cas9, various modifications of which have often been used in various experiments aimed to edit genomes. Using a technology called CRISPR/Cas9, researchers tried to clear the cells of rodents from HIV.
After checking the results obtained after two weeks, the researchers saw that the cells are lymphocytes and in lymph nodes the amount of viral RNA declined significantly. According to experts, this suggests that tested their method is potentially very effective.
Their work, scientists published in the journal Gene Therapy.Related posts: